Molecular and Modern Studies on Potato Leaf Roll Virus (Plrv): Viral & Tissue Culture Study - Emad Fathy Mohamed Dwidar - Books - LAP LAMBERT Academic Publishing - 9783659553448 - June 11, 2014
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Molecular and Modern Studies on Potato Leaf Roll Virus (Plrv): Viral & Tissue Culture Study


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This investigation was carried out to achieve the following: 1. Conducting some molecular studies on PLRV such as : ? Extraction of RNA ? Reverse transcriptiom reaction ? PCR ? Purification of PCR product ? Cloning of PCR product ? Large scale plasmid preparation(Maxi-prep) ? Subcloning ? Large scale plasmid preparation(Maxi-prep) 2. Using of modern serological methods for rapid detection of PLRV such as: ? ELISA ? DBIA ? TBIA 3. Recognize the intracellular changes induced by the PLRV using Electron Microscopy 4. Production of PLRV-free potato plants.

Media Books     Paperback Book   (Book with soft cover and glued back)
Released June 11, 2014
ISBN13 9783659553448
Publishers LAP LAMBERT Academic Publishing
Pages 152
Dimensions 150 × 220 × 10 mm   ·   244 g
Language German