Duplex Rt-pcr Assays for Rapid Detection,sorting of Influenza Viruses - Anish Somu - Books - LAP LAMBERT Academic Publishing - 9783659344961 - February 13, 2013
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Duplex Rt-pcr Assays for Rapid Detection,sorting of Influenza Viruses


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Future pandemic prevention from influenza virus type A strain (H1N1) 2009 in India require rapid and reliable testing of larger samples collected in year round at different geographical, environmental and social strata for novel strains and its differentiation from seasonal ones. In this study, a real-time Reverse Transcriptase PCR (RT-PCR) assay was used in multiplex for detecting Influenza A gene, Swine influenza H1 gene and human RNP gene, with normal PCR in singleplex for B viruses for 24 patient nasal swab samples, who were reporting with influenza like illness (ILI) from local clinic in Chennai and these patients showed low circulation of pandemic (H1N1) 2009 strain. KEYWORDS: Influenza, Nasal Swab, Real-Time PCR, RT-PCR, Swine Flu, (H1N1) 2009 Strain

Media Books     Paperback Book   (Book with soft cover and glued back)
Released February 13, 2013
ISBN13 9783659344961
Publishers LAP LAMBERT Academic Publishing
Pages 104
Dimensions 150 × 6 × 225 mm   ·   163 g
Language English