Alak Kanti Datta

Effect of Growth Regulators on Tomato (Lycopersicon Esculentum Mill.): on Shoot Induction and Plant Regeneration in Lycopersicon Esculentum Mill Alak Kanti Datta

Four locally grown varieties of tomato; BINA-3, BARI-3, Bahar, Maple and one Indian variety; Pussa Rubby (PR) were selected in this study to observe the effects of growth regulators(in different concentrations&combinations)on shoot initiation and root induction. MS media containing 1.5mg/l BAP and 0.2mg/l IAA showed best result with lowest callus formation and increased number of shoot in all the varieties. In this medium the best response was found for Maple(93.33%)followed by Pussa Ruby(86.67%)and for the other varieties such as BARI-3, BINA-3& Bahar gave almost similar response to that combination of the medium. The survival response of all varieties was in between 80-90%.. BINA-3 showed highest number of fruits per plant while BINA-3 and BARI-3 showed highest average number of seeds per fruit. Finally,it was found that 1.5 mg/l BAP with 0.2mg/l IAA containing MS media is better for shoots induction and 0.2mg/l IAA containing ½ strength MS media is better for root formation in all five varieties. Each experiment was conducted three times(n=3). Regeneration percentages were analyzed by using SPSS(version 16)& significant differences among means were assessed by the Duncan test(P<0.05).