Sherif Abdeen

New Trends in Large-scale Mammalian Cell Culture: Cell Adaptation: Studies on Hela Cell Growth in Serum-enriched and Serum-free Media (Sfm): High Suspension Cell Density in Production Sfm Sherif Abdeen

This book offers the experiences and improvements made in mammalian cell culture technology. It bridges two scientific cultures, Scientists with no background in cultivation of animal cells and for engineers with a little ground in biology. It was designed to provide you with immediate access to the protocol required every day for animal and mammalian cell lines cultures. It explains the advantages and disadvantages of the methods, different application and gives advice whichever procedure to use. The author supplements the ideas by simple worked examples, making the techniques of scaling up cells by adaptation strategy for pharmaceutical protein production easy to learn. Monitoring cell growth kinetics accompanied by cell metabolism (glucose and glutamine consumption, lactate and ammonia production) and cell imaging established well. It discusses removal of serum, media supplement like insulin and amino acids, osmotic stress and cell morphology. It worth mentioned that, this book open a new gate for biotechnological scientists describing manipulation of productive adherence cell factory in serum medium to grow as suspension cells in serum free medium at high cell density.