Capillary Electrophoresis: Methods and Potentials - Heinz Engelhardt - Books - Springer-Verlag Berlin and Heidelberg Gm - 9783642858567 - June 12, 2012
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Heinz Engelhardt
Capillary Electrophoresis: Methods and Potentials 1996 edition

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Capillary electrophoresis (CE), also designated by the acronym HPCE for "High Per­ formance Capillary Electrophoresis" is a rapidly growing analytical separation method. It unites the separation technique of classical electrophoresis on plates with the instrumental methods of chromatography with respect to direct detection of the solutes separated in the capillary and their ready identification and quantification. The initial problems of inadequate reproducibility in quantitative analysis, due to the necessity of handling extremely small volumes, have largely been solved in the sec­ ond generation commercial instruments. Hence, a rapid and reliable separation sys­ tem is available for ionic compounds from the smallest cation (the lithium ion) up to poly anions with molecular weights ranging in the millions (such as DNA molecules). The methods of gel electrophoresis and isoelectric focusing can be readily extended to separation techniques carried out in a capillary. For nonionic compounds an addi­ tional separation method is available in the form of micellar electrokinetic chroma­ tography (MEKC). This involves a true chromatographic separation process because the distribution of the analytes between the buffer and the micelles is superimposed on the electrophoretic migration, which contributes substantially to the selectivity.

225 pages, 19 black & white illustrations, biography

Media Books     Paperback Book   (Book with soft cover and glued back)
Released June 12, 2012
ISBN13 9783642858567
Publishers Springer-Verlag Berlin and Heidelberg Gm
Pages 215
Dimensions 170 × 244 × 12 mm   ·   367 g
Language English  
Translator Gutnikov, G.

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